Analysis Note

ControlRIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene

Application

Western Blot Analysis:
1-2 µg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.

Research Sub CategoryEpitope Tags

Anti-Neomycin Phosphotransferase II Antibody is an antibody against Neomycin Phosphotransferase II for use in WB.

Research CategoryEpitope Tags & General Use

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin^®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

Immunogen

Full length neomycin phosphotransferase (NPT) II

Legal Information

Geneticin is a registered trademark of Gibco BRL Life Technologies, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

Quality

Routinely evaluated by Western Blot on transiently transfected Cos-1 cells.

Western Blot Analysis:
1-2 µg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.

Specificity

Recognizes NPT II, Mr ~30 kDa

Storage and Stability

Stable for 1 year at 2 - 8°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2 - 8°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below 2 - 8°C may cause glycerol containing solutions to become frozen during storage.

Target description

~30 kDa